玉米赤霉烯酮对小鼠睾丸间质细胞内StAR蛋白及类固醇合成关键酶表达的影响

doi:10.11843/j.issn.0366-6964.2014.07.009

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (7): 1091-1096.doi: 10.11843/j.issn.0366-6964.2014.07.009

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Effects of Zearalenone on the Expression of Steroidogenic Acute Regulatory Protein and Key Enzymes in Primary Leydig Cells

ZHENG Wang-long^1,2，LIU Qing^1，3，WANG Ya-jun¹，HUANG Qin-yi¹，XU Hui¹，WANG Yi-an¹，GU Jian-hong¹，YUAN Yan¹，LIU Xue-zhong¹，LIU Zong-ping^1,2，BIAN Jian-chun^1,2*

（1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China; 2.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses，Yangzhou 225009,China; 3.Jiaozuo Entry-Exit Inspection and Quarantine Bureau,Jiaozuo 454001,China)

Received:2014-01-22 Online:2014-07-23 Published:2014-07-23

Abstract

Abstract:

In order to investigate the toxic mechanim of the inhibition of androgen secretion induced by ZEA (Zearalenone）in leydig cells，the primary leydig cells，isolated in vitro，were exposed to 0 (control group)，1，5，10，20 μg•mL^-1 ZEA for 12 h，respectively. Western-blot and QRT-PCR were used to measure the expressions of Steroidogenic acute regulatory protein（StAR）,P450scc,3β-HSD,P450c17a1 and 17β-HSD mRNA and the related proteins. The results showed that the transcription of the mRNA of StAR and the expression of the related proteins were increased dramatically (P<0.05，P<0.01) depended on the concentration of ZEA. In contrast，the transcription of the mRNA of P450scc,3β-HSD,P450c17a1 and 17β-HSD and the expression of the related proteins were decreased significantly (P<0.05,P<0.01).In conclusion，the key enzymes of steroid secretion in the pathway of testosterone synthesis played an important role in the inhibition of androgen secretion induced by ZEA (Zearalenone） in leydig cells.

CLC Number:

S865.1⁺3.3

ZHENG Wang-long，LIU Qing，WANG Ya-jun，HUANG Qin-yi，XU Hui，WANG Yi-an，GU Jian-hong，YUAN Yan，LIU Xue-zhong，LIU Zong-ping，BIAN Jian-chun. Effects of Zearalenone on the Expression of Steroidogenic Acute Regulatory Protein and Key Enzymes in Primary Leydig Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1091-1096.

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Effects of Zearalenone on the Expression of Steroidogenic Acute Regulatory Protein and Key Enzymes in Primary Leydig Cells

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